UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN LENGKUAS (Alpinia galanga L.) TERHADAP Escherichia coli
DOI:
https://doi.org/10.64788/ar-rasyid.v1i5.61Keywords:
Diarrhea, Escherichia coli, Galangal Leaf, ExtractAbstract
Diarrhea is caused by bacteria, one of which is Escherichia coli, which is still a serious health problem, especially due to increasing resistance to synthetic antibiotics. Efforts are being made to overcome this by looking for natural antibacterial agents from medicinal plants, one of which is galangal leaves (Alpinia galanga L.). This study aims to evaluate the antibacterial activity of ethanol extract of galangal leaves against Escherichia coli. This research used a true experimental method with concentration variations of 5%, 10% and 20%. The antibiotic ciprofloxacin was used as a positive control, and distilled water as a negative control. Galangal leaves were extracted using the maceration method with 70% ethanol solvent, and the antibacterial test was carried out using the disk disk method. Data analysis was carried out using one way ANOVA. The results showed that the ethanol extract of galangal leaves had significant antibacterial activity, with an average inhibition zone diameter of 13.72 mm (5%), 14.39 mm (10%), and 15.38 mm (20%). The results of all data were normally and homogeneously distributed with values > 0.05 and the ANOVA test results were 0.00 (p<0.05) which indicated there were significant differences between concentrations. Based on the classification of inhibitory power, antibacterial activity at a concentration of 20% is included in the strong category. This research indicates that the ethanol extract of galangal leaves has the potential to be a natural antibacterial agent against Escherichia coli.
Downloads
References
1. Rokhayati E, Dap YH. Hubungan sanitasi lingkungan dengan kejadian diare pada balita di Kecamatan Jebres Surakarta The. Heal Res. 2024;7(1):85–91. DOI: 10.36419/avicenna.v7i1.1032
2. Rahardjoputro. Ketepatan Pemilihan Peresepan Antibiotik untuk terapi Pneumonia pada Pasien Lansia Rawat Inap di Rumah Sakit X Surakarta. Indones J Pharm Nat Prod. 2023;6(01):1–7. DOI: http:/jurnal.unw.ac.id/index.php/ijpnp
3. Sumampouw OJ. Uji sensitivitas antibiotik terhadap bakteri Escherichia Coli penyebab diare balita di Kota Manado (The Sensitivity Test of Antibiotics to Escherichia coli was caused the diarhhea on underfive children in Manado City). J Curr Pharm Sci. 2018;2(1):105. DOI: journal.umbjm.ac.id/index.php/jcps
4. Mambang. Uji farmakognostik dan identidikasi senyawa pada beberapa tingkatan fraksi ekstrak etanol daun Lengkuas (Alpinia Galanga). J Pharm Care Sci. 2020;1(1):102–10. DOI: 10.33859/jpcs.v1i1.27
5. Asri. Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Shigella dysenteriae. LenteraBio. 2022;11(3):441–8. DOI: https://doi.org/10.26740/lenterabio.v11n3.p441-448
6. Fajrul. Uji daya hambat ekstrak etanol daun lengkuas (Alpinia galanga L.) tehadap bakteri Streptococcus mutans. J Inov Farm Indones. 2024;5(2):87–93. DOI : https://doi.org/10.30737/jafi.v5i2.442573
7. Aini RN, Listyani TA, Raharjo D. Perbandingan kadar flavonoid dan aktivitas antioksidan ekstrak etanol dan infusa daun rambutan (Nephelium lappaceum L.) Dengan metode ABTS. J Ilm Wahana Pendidikan, Desember [Internet]. 2023;9(23):665–80. Available from: https://doi.org/10.5281/zenodo.10353550.
8. Nahor EM, Rumagit BI, Tou HY. Perbandingan rendemen ekstrak etanol daun andong (Cordyline futicosa L.) menggunakan metode ekstraksi maserasi dan sokhletasi. semin Nas Tahun 2020. 2020;40–4. DOI: https://ejurnal.poltekkesmanado.ac.id/index.php/prosiding2020/article/view/1367/902
9. Sukadiasa. Uji efektivitas antibakteri ekstrak etanol 96% tanaman gonda (Sphenoclea zeylanica Gaertn) terhadap Staphylococcus aureus. J Ilm Medicam. 2023;9(1):61–9. DOI: 10.36733/medicamento.v9i1.4644
10. Asmaliani. Uji aktivitas antibakteri ekstrak etanol daun gaharu (Aquilaria malaccensis) dengan menggunakan metode klt-bioautografi. Makassar pharm sci j [Internet]. 2024;1(31):281–94. Available from: https://journal.farmasi.umi.ac.id/index.php/mpsj
11. Qur’aniati et al, I. G. A. Manik Widhyastini DS. Inhibitory capacity of clay mask 96% Ethanol Extract From Bitter Melon (Momordica charantia L.) against Staphylococcus aureus. Sains Nat. 2022;12:124–33. DOI: https://doi.org/10.31938/jsn.v 12i3.413
12. Rizki SA, Latief M, Rahman H. Uji aktivitas antibakteri ekstrak n-heksan, etil asetat dan etanol daun durian (Durio zibethinus Linn.) terhadap bakteri Propionibacterium acnes dan Staphylococcus epidermidis. J Mhs Farm. 2021;442–57. DOI: https://onlinejournal.unja.ac.id/kedokteran/article/view/14668
13. Khasanah NI, Fatwami EF, Royani S. Skrining fitokimia dan evaluasi fisik sediaan facial wash gel ekstrak daun sirsak (Annona muricata L.). J Pharmacopolium. 2023;6(3):76–83. DOI: 10.36465/jop.v6i3.1200
14. Ariya. Pengaruh jumlah pelarut terhadap rendemen ekstrak daun katuk (Sauropus Androgynus L. Merr). Sitawa J Farm Sains dan Obat Tradis. 2022;1(2):125–35. DOI: 10.62018/sitawa.v1i2.22
15. Maheshwaran L, Nadarajah L, Senadeera SPNN, Ranaweera CB, Chandana AK, Pathirana RN. Phytochemical Testing Methodologies and Principles for Preliminary Screening/ Qualitative Testing. Asian Plant Res J. 2024;12(5):11–38. DOI:10.9734/aprj/2024/v12i5267
16. Wulandari G. Uji aktivitas antibakteri ekstrak etanol kulit buah alpukat (Persea americana Mill) terhadap bakteri Staphylococcus aureus. Media Inf. 2019;15(1):74–80. DOI: 10.37160/bmi.v15i1.229
17. Suprobo CO, Suprihati, Wuryanti. Jurnal Kimia Sains dan Aplikasi. 2010;13(2):51–6. Available from:https://scholar.archive.org/work/forwjldoafbenilgbyc4sxum2m/access/wayback/http://ejournal.undip.ac.id:80/index.php/ksa/article/viewFile/15913/11854
18. Kalalo WM, Purwanjani W, An U, Purwodadi N, Tengah J. Antibacterial Activity Test on Face Toner Preparations 70 % Ethanol Extract of Avocado Leaves ( Persea americana Mill ) Against Staphylococcus Aureus Bacteria. 2024;3(1):61–77.
19. Ferdinan A, Rizki FS, Kurnianto E, Kurniawan K. Fraksinasi dan identifikasi senyawa tanin dari ekstrak pandan hutan (Freycinetia sessiliflora Rizki). J Borneo. 2022;2(2):93–8. DOI: 10.57174/jborn.v2i2.48
20. Firawati M, Iqbal Pratama. Isolasi dan identifikasi senyawa saponin daun bungkus (Smilax rotundifolia) menggunakan metode spektrofotometri Ultraviolet. Firawati M Iqba Pratama [Internet]. 2018;6(2):115–21. Availablefrom:https://journal.uinalauddin.ac.id/index.php/jurnal_farmasi/article/download/6757/5432
21. Waluyo. Daya hambat ekstrak etanol daun akasia berduri(acacia nilotica l.) Terhadap pertumbuhan bakteri streptococcus pneumoniae Seminar nasional pendidikan. Manaj Konflik Dalam Organ. 2016;1(0711):568–75. DOI: https://www.neliti.com/publications/138879/aya-hambat-ekstrak-etanol-daun-akasia-berduri-acacia-nilotica-l-terhadap-pertumb
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Putri Ema Hartanty, Na’imatul Retno Faizah, Octaviana Dyah Oentari (Author)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
