Uji AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEMUNING (Murraya paniculata (L.) Jack) TERHADAP BAKTERI Streptococcus pneumoniae
DOI:
https://doi.org/10.64788/ar-rasyid.v1i5.51Keywords:
orange jasmine leaf extract, pneumonia, Streptococcus pneumoniaeAbstract
One of the main infectious causes of death in Indonesia is pneumonia, or inflammation of the lungs, which is brought on by a number of germs, including the bacteria Streptococcus pneumoniae. The objective of this study is to ascertain the ideal concentration at which antibacterial activity is present and to assess the antibacterial activity of ethanol extracts of kemuning leaves (Murraya paniculata (L.) Jack) against Streptococcus pneumoniae at concentrations of 15%, 20%, and 25%. The disc diffusion method was used to perform the antibacterial test after kemuning leaves were extracted using the maceration procedure with 70% ethanol. One-way ANOVA was used to analyse the data. With the highest inhibition zone at a concentration of 25% (10.31 ± 0.58 mm), classed as strong, and concentrations of 15% (9.83 ± 0.39 mm) and 20% (9.64 ± 0.73 mm) classified as moderate, the results demonstrated that the ethanol extract of kemuning leaves exhibited significant antibacterial activity. The one-way ANOVA test results indicated that the concentrations differed significantly (p < 0,05). According to this study, kemuning leaf extract has the potential to be an antibacterial agent against Streptococcus pneumoniae.
Downloads
References
1. Rahayu AL, Darmawan G, Jaya IGNM. Calculation of the Risk Index for Diarrhea, ISPA, and Pneumonia in Toddlers in the City of Bandung Using Geographically Weighted Principal Component Analysis. Indones J Adv Res. 2023;2(4):285–300.
2. Setiawan RD. Profil Pasien Anak Balita Dengan Pneumonia Di Rspal Dr. Ramelan Surabaya Periode Januari 2021 - Januari 2022. Surabaya Biomed J. 2024;3(3):163–71.
3. Rahardjoputro R, Sholihah I, Amrullah AW. Ketepatan Pemilihan Peresepan Antibiotik untuk Terapi Pneumonia pada Pasien Lansia Rawat Inap di Rumah Sakit X Surakarta. Indones J Pharm Nat Prod. 2023;6(01):1–7.
4. Bimantara B. Efektivitas Tanaman Herbal Terhadap Pertumbuhan Bakteri Streptococcus pneumoniae. J Kesehat Alam. 2020;13:200–8.
5. Dwi K. Profil Kromatogram dan Aktivitas Antibakteri Ekstrak Etanol Daun Kemuning (Murraya paniculata (L.) Jack) Terhadap Escherichia coli In Vitro. J Sains Farm Klin. 2019;6:208–26.
6. Sabilla TC, Asep S. Efektivitas Penggunaan Ekstrak Kemuning (Murraya paniculata (L.) Jack) sebagai Antimikroba. Agromedicine J. 2019;6(20):320–4.
7. Gautam MK, Gangwar M, Nath G, Rao C V., Goel RK. In-Vitro Antibacterial Activity On Human Pathogens And Total Phenolic, Flavonoid Contents of Murraya paniculata Linn. Leaves. Asian Pac J Trop Biomed [Internet]. 2017;2(3 SUPPL.):S1660–3. Available from: http://dx.doi.org/10.1016/S2221-1691(12)60472-9
8. Dwi Puspitasari A, Proyogo LS. Perbandingan Metode Ekstraksi Maserasi dan Sokletasi terhadap Kadar Flavonoid Total Ekstrak Daun Kersen (Muntingia calabura). J Ilm Cendekia Eksakta. 2020;16–23.
9. Nobel G. Uji Efek Tonikum Kombinasi Ekstrak Etanol Buah Cabe Jawa (Piper retrofractum Vahl.) Dan Madu Randu Terhadap Mencit Swiss Webster. J Phamaceutical Sci. 2024;4(1):1–23.
10. Monica Sandy, Siska Wardani T, Dwi Septiarini A. Uji Aktivitas Antibakteri Ekstrak, Fraksi N-Heksan, Fraktsi Etil Asetat, Fraksi Air Daun Pegagan (Centella asiatica (L.) Urb) Terhadap Escherichia coli ATCC 25922. Media Farm Indones. 2021;16(2):1683–92.
11. Anggreany RT, Rahmawati I, Leviana F. Uji Antibakteri Ekstrak Dan Fraksi Herba Ceplukan (Physalis angulata L.) Untuk Mengatasi Infeksi Staphylococcus epidermidis Selama Persalinan. Din Kesehat J Kebidanan Dan Keperawatan. 2020;11(1):253–63.
12. Sari RP, Laoli MT. Karakterisasi Simplisia dan Skrining Fitokimia Serta Analisis Secara KLT (Kromatografi Lapis Tipis) Daun Kulit Buah Jeruk Lemon (Citrus limon (L.) Burm.f.). J Ilm Farm Imelda. 2019;2(2):59–68.
13. Malik A, Edward F, Waris R. Skrining Fitokimia dan Penetapan Flavonoid Total Ekstrak Metanolik Herba Boroco (Celosia argentea L.). J Fitofarmaka Indones. 2019;1(1):1–5.
14. Noviyanty Y, Hepiyansori, Marlina R. Identifikasi Senyawa Flavonoid Dari Ekstrak Etanol Kulit Buah Jeruk Kalamansi (Citrus x microcarpa Bunge). J Ilm Farm. 2019;11(1):1–14.
15. Kalalo WM, Purwanjani W, An U, Purwodadi N, Tengah J. Antibacterial Activity Test on Face Toner Preparations 7 % Ethanol Extract of Avocado Leaves (Persea americana Mill) Against Staphylococcus aureus Bacteria. Pratama Med J Kesehat. 2024;3(1):61–77.
16. Banu R, Nagarajan N. TLC and HPTLC Fingerprinting Of Leaf Extracts of Wedelia Chinensis (Osbeck) Merrill. J Pharmacogn Phytochem. 2016;2(6):29–33.
17. Rachman A, Wardatun S, Weandarlina IY. Isolasi dan Identifikasi Senyawa Saponin Ekstrak Metanol Daun Binahong (Anredera cordifolia (Ten.) Steenis). J Farm. 2018;4(5):3–8.
18. Qur’aniati S, Widhyastini IGAM, Susanty D. Inhibitory Capacity of Clay Mask 96% Ethanol Extract from Bitter Melon (Momordica charantia L.) Against Staphylococcus aureus. J Sains Nat. 2022;12(3):124.
19. Sutomo S, Hasanah N, Arnida A, Sriyono A. Standardisasi Simplisia dan Ekstrak Daun Matoa (Pometia pinnata J.R Forst & G. Forst) Asal Kalimantan Selatan. J Pharmascience. 2021;8(1):101.
20. Kemenkes RI. Farmakope Herbal Indonesia Edisi II. Kementrian Kesehatan RI. Jakarta; 2017. 97–103 p.
21. Dewatisari WF, Rumiyanti L, Rakhmawati I. Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. J Penelit Pertan Terap. 2017;17(3):197.
22. Lilyawati SA, Fitriani N, Prasetya F. Aktivitas Antimikroba Ekstrak Etanol Biji Pinang Muda (Areca catechu). Proceeding Mulawarman Pharm Conf. 2019;10:135–8.
23. Ningrum IS. Uji Pengaruh Seduhan Simplisia Daun Kemuning (Murraya paniculata (L.) Jack) Terhadap Pertumbuhan Bakteri Salmonella typhi. Public Heal J. 2017;4.
24. Harborne JB. Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. In 2020.
25. Kusumo GG, Ferry Fernanda MAH, Asroriyah H. Identifikasi Senyawa Tanin Pada Daun Kemuning (Murraya paniculata L. Jack) Dengan Berbagai Jenis Pelarut Pengekstraksi. J Pharm Sci. 2019;2(1):29–32.
26. Marliana SD, Suryanti V, Suyono. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol. Biofarmasi. 2016;3(1):26–31.
27. Hafizah DA, Sunardi. Pemisahan Kromatografi Lapis Tipis pada Asam Amino dengan Menentukan Nilai Faktor Retensi. J Kim dan Rekayasa. 2024;5.
28. Azizy ZAB. Optimasi Proses Ekstraksi Senyawa Metabolit Sekunder Dari Tanaman Daun Afrika (Vernonia amygdalina) Secara Microwave-Assisted Extraction. J Mhs UNHAS. 2021;9–10.
29. Lipsy P. Thin Layer Chromatography Characterization of the Active Ingredients in Excedrin and Anacin. Chem Chem J. 2016;
30. Khasanah K, Rusmalina S, Safira D, Setyorini EA, Amanah N. Penerapan Green Chemistry Pada Deteksi Kandungan Pewarna Berbahaya (Rhodamin B) Pada Produk Kosmetik Yang Beredar di Wilayah Pekalongan. Pena J Ilmu Pengetah dan Teknol. 2022;36:25.
31. Handayani R, Qamariah N, Mardova SA. Uji Daya Hambat Ekstrak Etanol Batang Saluang Belum Terhadap Bakteri Escherichia coli. J Borneo Farm. 2019;1(1):16–8.
32. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay. Appl Microbiol. 2019;22(4):666–70.
33. Tahir DNF. Uji Aktivitas Antibakteri Ekstrak Daun Kemuning (Murraya paniculata (L.) Jack) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. J biosainsdik. 2019;
Downloads
Published
Issue
Section
License
Copyright (c) 2025 El Santi Sadida, Na’imatul Retno Faizah, Octaviana Dyah Oentari (Author)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
